Dibutyltin Dichloride (DBTC)-Induced Pancreatitis Model
At Creative Bioarray, we have accumulated extensive knowledge and expertise in conducting pancreatitis studies. Our team of experts has established a highly effective pancreatitis model induced by dibutyltin dichloride (DBTC), which enables us to evaluate the efficacy of test compounds in treating pancreatitis. With our advanced model and state-of-the-art facilities, we are committed to providing our clients with accurate and reliable results for their research projects related to pancreatitis.
DBTC is a kind of fat-soluble substance, by the liver, and gallbladder drainage to the pancreatic duct, causing cell necrosis and epithelial hyperplasia of the pancreatic duct, and obstruction of the pancreatic duct. DBTC causes CP in rats via a single intravenous injection. DBTC induces acute pancreatic inflammation within 24 hours, which progresses to chronic inflammation in a week, and then to progressive fibrotic lesions over the following 2 months, with chronic and acute inflammation (mediated by T cells and macrophages). This model has become widely used in research on the pathogenesis of pancreatitis and drug efficacy analysis due to its low modeling cost and simple methodology.
Our Dibutyltin Dichloride (DBTC) Induced Pancreatitis Model
- Animal Available
Rat - Modeling Method
At Creative Bioarray, we induce the pancreatitis model by tail vein injection of DBTC. - Group Setting
- Control group
- Model group
- Three dose groups of test compounds
- Endpoints
- Images of pancreas
- Body weight
- Food intake
- Histology analysis: H&E staining, Sirius red staining
- Cytokine analysis: TNF-α, IL-1β, IL-6, etc.
- Serum analysis: Amylase, Lipase, etc.
- qPCR and Western Blot
- Other customized endpoints: available upon request
Example Data
Fig. 1 The pathological score of H and E staining (original magnification, ×200)
Fig. 2 Serum amylase levels and lipase levels
Additionally, we also offering other pancreatitis models maybe you interested:
Quotation and Ordering
Creative Bioarray, a trusted provider of disease models, is dedicated to providing guidance for your research. Our team of specialists can work with you to custom a detailed study design to meet your specific needs. If you are interested in our services, please do not hesitate to contact us at any time or submit an inquiry to us directly.
Reference
- Li, C., et al. Saikosaponin D attenuates pancreatic injury through suppressing the apoptosis of acinar cell via modulation of the MAPK signaling pathway. Frontiers in Pharmacology, 2021, 12: 735079.
